Preheat the air fryer to 350°F.
Whisk together the sour cream, lime juice, chili powder, garlic powder, salt, and black pepper in a small bowl.
Spray the cooking rack in the air fryer with cooking spray. Spread the chili lime sauce on one tortilla and place it sauce-side up in the air fryer.
Arrange the chicken and cheese on top of the sauce, cover it with the second tortilla, and spray the top with cooking spray.
Secure the quesadilla with 2 or 3 toothpicks by threading them through the edges of the two tortillas.
Air fry for 8 minutes, flipping halfway through.
Remove and let it cool before slicing into fourths and serving with guacamole and pico de gallo.