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Preheat the air fryer to 350°F.
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Whisk together the sour cream, lime juice, chili powder, garlic powder, salt, and black pepper in a small bowl.
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Spray the cooking rack in the air fryer with cooking spray. Spread the chili lime sauce on one tortilla and place it sauce-side up in the air fryer.
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Arrange the chicken and cheese on top of the sauce, cover it with the second tortilla, and spray the top with cooking spray.
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Secure the quesadilla with 2 or 3 toothpicks by threading them through the edges of the two tortillas.
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Air fry for 8 minutes, flipping halfway through.
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Remove and let it cool before slicing into fourths and serving with guacamole and pico de gallo.